Isolation of mitochondria from cell cultures by PCT for proteomic analysis.

Proteomic profiling of mitochondria has the potential to provide insights into mitochondrial functions associated with aging, various metabolic states, and diseases such as cancer, diabetes, and cardiovascular disease [1]. Rapid and reproducible isolation of intact mitochondria is crucial for efficient enrichment and subsequent proteomic analysis of low-abundance mitochondrial proteins [2]. Here we describe a system for the isolation of intact mitochondria from rat PC12 cells using pressure cycling technology (PCT).